Molecular Weight:Article Title: SmallTalk: a novel small-sized fusion tag for peptide expression and purification.
Article Snippet: Gels were run in 19 standard Tris-Glycine SDS running buffer, prepared from a 109 stock solution that contains 0.25 M Tris base, 1.92 M Glycine, and 1% SDS, using a mini-vertical electrophoresis unit (Mini-PROTEAN system; Bio-Rad Laboratories, Hercules, CA, USA). .. For the molecular weight estimation of small-scale expressions of SmallTalk-GFP, an unstained protein standard with a broad range (10–200 kDa, New England Biolabs) was used, as shown in Fig. 4. ..
Article Title: SmallTalk : a novel small‐sized fusion tag for peptide expression and purification
Article Snippet: Gels were run in 1× standard Tris‐Glycine SDS running buffer, prepared from a 10× stock solution that contains 0.25 m Tris base, 1.92 m Glycine, and 1% SDS, using a mini‐vertical electrophoresis unit (Mini‐PROTEAN system; Bio‐Rad Laboratories, Hercules, CA, USA). .. For the molecular weight estimation of small‐scale expressions of SmallTalk‐GFP, an unstained protein standard with a broad range (10–200 kDa, New England Biolabs) was used, as shown in Fig. . ..
SDS Page:Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan-Specific Antibodies.
Article Snippet: E. coli BL21-CodonPlus (DE3)-RIL competent cells (Agilent, cat. no. 230245) Plasmid DNA 2×YT medium (see recipe): without antibiotics Duraivelan et al. 7 of 37 Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense containing 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose containing 50 μg/ml kanamycin only 1 M IPTG (see recipe) scFv-H resuspension buffer (see recipe) 50% (v/v) His60 slurry (see recipe), freshly prepared His60 wash buffer (see recipe) His60 elution buffer (see recipe) 4× Laemmli buffer (Bio-Rad, cat. no. 1610747) Dithiothreitol (DTT, Gold Bio, cat. no. DTT100) Criterion XT precast gels, 4-12% Bis-Tris (Bio-Rad, cat. no. 3450125) Unstained protein standard (New England Biolabs, cat. no. P7717S) SDS-PAGE running buffer (see recipe) SDS-PAGE staining solution (see recipe) LB agar plates (see recipe), 8 wells, with 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose 10% (v/v) antifoam solution (see recipe) scFv-H SEC running buffer (see recipe) Gel filtration standards (Bio-Rad, cat. no. 1511901) 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- A B 0 25 50 75 100 125 150 0 400 800 1200 0 25 50 75 100 Elution volume (ml) A 28 0 (m A U) A 280 (m A U) Standard HS001H 69.31 ml | -1 .3 5 kD a -1 7 kD a -4 4 kD a -1 58 kD a -6 70 kD a 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 96-well PCR plate (Thermo Fisher Scientific, cat. no. AB2396) 42°C heat block or thermocycler for 96-well plate 96-well 2-ml deep-well blocks (USA Scientific, cat. no. 1896-2000), sterilized by autoclaving at 121°C for 15 min AeraSeal breathable seals (Excel Scientific, cat. no. B100) Bacterial shaking incubator at 37° and 22°C (e.g., Shel Lab S16R-HS-2 for small scale, New Brunswick Scientific I 26 for large scale) Spectrophotometer (e.g., Stunner, Unchained Labs) Bacterial plate incubator (e.g., Shel Lab S16R-HS-2) at 37°C Plate shaker (e.g., Ika MTS 2/4) at 4°C 96-well, 0.22-μm filter plate (Thomson, cat. no. 931919) Vacuum manifold for 96-well filter plates (e.g., Sigma, cat. no. 57192-U) SDS-PAGE gel running equipment (Bio-Rad, cat. no. 1656019) Gel imaging system (e.g., FluorChem, ProteinSimple) 250-ml Erlenmeyer flasks (VWR, cat. no. 76531-762) 2-L baffled flasks (Genesis Pyrex, cat. no. 4446-2L) 1-L centrifuge bottles (Beckman, cat. no. C31597) 50-ml centrifuge tubes (Fisher Scientific, cat. no. 14955239) Rocker 20-ml Econo-Pac chromatography columns (Bio-Rad, cat. no. 7321010) 0.45-μm syringe filters (Genesee Scientific, cat. no. 25-242) FPLC system for protein purification (e.g., GE ÄKTA) equipped with HiLoad 16/600 Superdex 75-pg column (Cytiva, cat. no. 28989333) 10-kDa centrifugal concentrators (Amicon Ultra-15, Millipore, cat. no. UFC901024) Perform small-scale expression test Day 0: Transform cells and prepare inoculum 1.
Article Title: High‐Fidelity In Vitro Packaging of Diverse Synthetic Cargo into Encapsulin Protein Cages
Article Snippet: .. Unstained Protein Standard, Broad Range 10-200 kDa (New England Biolabs) was used as the ladder for all SDS-PAGE gels. .. Blue Native PAGE was conducted using NativePAGE 3-12% Bis-Tris 1.0 mm Mini Protein Gels (ThermoFisher Scientific).
Staining:Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan-Specific Antibodies.
Article Snippet: E. coli BL21-CodonPlus (DE3)-RIL competent cells (Agilent, cat. no. 230245) Plasmid DNA 2×YT medium (see recipe): without antibiotics Duraivelan et al. 7 of 37 Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense containing 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose containing 50 μg/ml kanamycin only 1 M IPTG (see recipe) scFv-H resuspension buffer (see recipe) 50% (v/v) His60 slurry (see recipe), freshly prepared His60 wash buffer (see recipe) His60 elution buffer (see recipe) 4× Laemmli buffer (Bio-Rad, cat. no. 1610747) Dithiothreitol (DTT, Gold Bio, cat. no. DTT100) Criterion XT precast gels, 4-12% Bis-Tris (Bio-Rad, cat. no. 3450125) Unstained protein standard (New England Biolabs, cat. no. P7717S) SDS-PAGE running buffer (see recipe) SDS-PAGE staining solution (see recipe) LB agar plates (see recipe), 8 wells, with 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose 10% (v/v) antifoam solution (see recipe) scFv-H SEC running buffer (see recipe) Gel filtration standards (Bio-Rad, cat. no. 1511901) 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- A B 0 25 50 75 100 125 150 0 400 800 1200 0 25 50 75 100 Elution volume (ml) A 28 0 (m A U) A 280 (m A U) Standard HS001H 69.31 ml | -1 .3 5 kD a -1 7 kD a -4 4 kD a -1 58 kD a -6 70 kD a 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 96-well PCR plate (Thermo Fisher Scientific, cat. no. AB2396) 42°C heat block or thermocycler for 96-well plate 96-well 2-ml deep-well blocks (USA Scientific, cat. no. 1896-2000), sterilized by autoclaving at 121°C for 15 min AeraSeal breathable seals (Excel Scientific, cat. no. B100) Bacterial shaking incubator at 37° and 22°C (e.g., Shel Lab S16R-HS-2 for small scale, New Brunswick Scientific I 26 for large scale) Spectrophotometer (e.g., Stunner, Unchained Labs) Bacterial plate incubator (e.g., Shel Lab S16R-HS-2) at 37°C Plate shaker (e.g., Ika MTS 2/4) at 4°C 96-well, 0.22-μm filter plate (Thomson, cat. no. 931919) Vacuum manifold for 96-well filter plates (e.g., Sigma, cat. no. 57192-U) SDS-PAGE gel running equipment (Bio-Rad, cat. no. 1656019) Gel imaging system (e.g., FluorChem, ProteinSimple) 250-ml Erlenmeyer flasks (VWR, cat. no. 76531-762) 2-L baffled flasks (Genesis Pyrex, cat. no. 4446-2L) 1-L centrifuge bottles (Beckman, cat. no. C31597) 50-ml centrifuge tubes (Fisher Scientific, cat. no. 14955239) Rocker 20-ml Econo-Pac chromatography columns (Bio-Rad, cat. no. 7321010) 0.45-μm syringe filters (Genesee Scientific, cat. no. 25-242) FPLC system for protein purification (e.g., GE ÄKTA) equipped with HiLoad 16/600 Superdex 75-pg column (Cytiva, cat. no. 28989333) 10-kDa centrifugal concentrators (Amicon Ultra-15, Millipore, cat. no. UFC901024) Perform small-scale expression test Day 0: Transform cells and prepare inoculum 1.
Filtration:Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan-Specific Antibodies.
Article Snippet: E. coli BL21-CodonPlus (DE3)-RIL competent cells (Agilent, cat. no. 230245) Plasmid DNA 2×YT medium (see recipe): without antibiotics Duraivelan et al. 7 of 37 Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense containing 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose containing 50 μg/ml kanamycin only 1 M IPTG (see recipe) scFv-H resuspension buffer (see recipe) 50% (v/v) His60 slurry (see recipe), freshly prepared His60 wash buffer (see recipe) His60 elution buffer (see recipe) 4× Laemmli buffer (Bio-Rad, cat. no. 1610747) Dithiothreitol (DTT, Gold Bio, cat. no. DTT100) Criterion XT precast gels, 4-12% Bis-Tris (Bio-Rad, cat. no. 3450125) Unstained protein standard (New England Biolabs, cat. no. P7717S) SDS-PAGE running buffer (see recipe) SDS-PAGE staining solution (see recipe) LB agar plates (see recipe), 8 wells, with 34 μg/ml chloramphenicol, 50 μg/ml kanamycin, and 2% (w/v) glucose 10% (v/v) antifoam solution (see recipe) scFv-H SEC running buffer (see recipe) Gel filtration standards (Bio-Rad, cat. no. 1511901) 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- A B 0 25 50 75 100 125 150 0 400 800 1200 0 25 50 75 100 Elution volume (ml) A 28 0 (m A U) A 280 (m A U) Standard HS001H 69.31 ml | -1 .3 5 kD a -1 7 kD a -4 4 kD a -1 58 kD a -6 70 kD a 15- 20- 25- 30- 40- 50- 60- 70- 85- 100- 150- 200- 10- Current Protocols 26911299, 2026, 5, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70358 by N at Prov Indonesia, W iley O nline L ibrary on [14/05/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 96-well PCR plate (Thermo Fisher Scientific, cat. no. AB2396) 42°C heat block or thermocycler for 96-well plate 96-well 2-ml deep-well blocks (USA Scientific, cat. no. 1896-2000), sterilized by autoclaving at 121°C for 15 min AeraSeal breathable seals (Excel Scientific, cat. no. B100) Bacterial shaking incubator at 37° and 22°C (e.g., Shel Lab S16R-HS-2 for small scale, New Brunswick Scientific I 26 for large scale) Spectrophotometer (e.g., Stunner, Unchained Labs) Bacterial plate incubator (e.g., Shel Lab S16R-HS-2) at 37°C Plate shaker (e.g., Ika MTS 2/4) at 4°C 96-well, 0.22-μm filter plate (Thomson, cat. no. 931919) Vacuum manifold for 96-well filter plates (e.g., Sigma, cat. no. 57192-U) SDS-PAGE gel running equipment (Bio-Rad, cat. no. 1656019) Gel imaging system (e.g., FluorChem, ProteinSimple) 250-ml Erlenmeyer flasks (VWR, cat. no. 76531-762) 2-L baffled flasks (Genesis Pyrex, cat. no. 4446-2L) 1-L centrifuge bottles (Beckman, cat. no. C31597) 50-ml centrifuge tubes (Fisher Scientific, cat. no. 14955239) Rocker 20-ml Econo-Pac chromatography columns (Bio-Rad, cat. no. 7321010) 0.45-μm syringe filters (Genesee Scientific, cat. no. 25-242) FPLC system for protein purification (e.g., GE ÄKTA) equipped with HiLoad 16/600 Superdex 75-pg column (Cytiva, cat. no. 28989333) 10-kDa centrifugal concentrators (Amicon Ultra-15, Millipore, cat. no. UFC901024) Perform small-scale expression test Day 0: Transform cells and prepare inoculum 1.
other:Article Title: Characterization of a C-methyltransferase from Streptomyces griseoviridis – crystal structure, mechanism, and substrate scope
Article Snippet: Ref. = Unstained Protein Standard, Broad Range (10-200 kDa), New England Biolabs.
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